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KMID : 0361019920350010203
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Korean Journal of Otolaryngology - Head and Neck Surgery
1992 Volume.35 No. 1 p.203 ~ p.212
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Central Projections of the Vestibular Nerve in Chinchilla: 1. Scratch Method-an Extracellular Labelling Technique
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이원상/V. Honrubia
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Abstract
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A new method has been developed to label primary afferent fibers in the vestibular nerve with the use of extracellular applied horseradish peroxidase(HRP) in the inner ear space. Only the fibers innervating organs whose sensory receptor areas are
mechanically damaged take up and transport HRP onto the brain stem.
In 32 chinchillas, vestibular nerve labelling was performed successfully in all animals by scratching the surface with an electrolytically sharpened needle and replacing the fluid in the vestibule with 30% HRP solution. The animals were incubated
for 48
hours prior to Diaminobenzidine(DAB) processing. This was found to be the optimal time for labelling the fibers projecting into the vestibular nuclei.
Only fibers innervation the damaged receptors were labelled Simply replacing the vestibular fluid with HRP did not label the ganglion cell nor the afferent fibers in the brain stem. The specificity of the labelling was verified by histological
inspection of the ganglion cells and nerve fibres innervating the damaged and intact receptors.
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